- Semi-quantitative analysis.
- Amplification range of 100-500 bp.
- Includes TP-PCR systems to detect described expansions leading to amplicon sizes >500 bp.
- Includes a positive control at the optimal amplification concentration, in which normal alleles are present for all assays performed with the kit, to demonstrate the functionality of the prepared amplification reactions.
- Designed and validated with the same PCR program as related products for the analysis of expansions involved in other neurodegenerative diseases, so they can be analyzed together.
- It complies with the quality requirements specified by ISO 13845 and ISO 14001 in relation to the materials used in its manufacturing process.
- For in vitro diagnostic use.
Description
Detection of expansions associated with spinocerebellar ataxias and other cerebellar ataxias (SCA1, SCA2, SCA3, SCA6, SCA7, SCA12, SCA17 and DRPLA) related to neurodegenerative diseases
Format
12 x 8 rxn
Technology
Fragment Analysis
Reference
IMG-152
Compatible equipment
3730xl DNA Analyzer (ThermoFisher Scientific)
Imegen-SCAs allows the analysis of CAG or CAG/CAA triplet expansions associated with cerebellar ataxias by means of conventional PCR and capillary electrophoresis. In addition, the kit offers a TP-PCR (triple repeat primed PCR) system to detect expansions of SCA2 and SCA7 not detectable with conventional PCR.
- Amount of DNA needed: 50 ng for each amplification system
- Sample type: Peripheral blood (Germinal)
- Number of reactions per sample: 10 (8 PCR and 2 TP-PCR)
- Number of targets: 8 (SCA1, SCA2, SCA3, SCA6, SCA7, SCA12, SCA17 and DRPLA)
- Manual work time: 45 minutes
- Duration of the PCR/TP-PCR program: 2 h 30 min
- Compatible thermal cyclers: Any conventional thermal cycler
- Sequencing with polymers compatible with the label 6-Carboxyfluorescein (6-FAM).
Imegen-SCAs
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