Imegen-SARS-CoV-2 Strain Typing

Multiplex detection of SARS-CoV-2 coronavirus and discrimination of B.1.128 and B.1.351 strains by real-time rRT-PCR

96 rxn

Reverse transcription in real time


7500 FAST Real-Time PCR System (ThermoFisher), QuantStudio 5 Real-Time PCR System (ThermoFisher), CFX96 Real-Time PCR cycler (BioRad)

  • Multiplexed qualitative assay that allows the specific detection of strains of epidemiological interest South Africa (B.1.351) with the VIC fluorophore and Brazil (B.1.1.28 (P1)) with the FAM fluorophore. In addition, the assay allows detection by means of the TexasRed fluorophore of the Orf1ab gene common to all identified strains of SARS-CoV 2.
  • The Orf1ab gene of SARS-CoV 2, allows to evaluate the process of retrotranscription and amplification of the extracted RNA.
  • Detection limit: Less than 100 copies per reaction for all targets tested.
  • Cross reactivity: Diagnostic evaluation with human pathological samples confirm the specificity of the Kit against the strains of interest.
  • SARS-CoV-2 genomic inclusivity: 100%.
  • It complies with the quality requirements specified by ISO 9001 and ISO 14001 in relation to the materials used in its manufacturing process.

Imegen® SARS-CoV-2 Strain Typing is a real-time reverse transcription PCR test intended for the qualitative detection of viral RNA from South African and Brazilian strains of SARS-CoV-2, in individuals where SARS-CoV-2 has been previously detected CoV-2.

  • Type of sample: RNA purified from respiratory samples
  • Amount of sample required: 6 µL
  • Number of reactions per sample: 1
  • Number of targets: 3
  • Manual work time: 10 – 30 min
  • Duration of the rRT-PCR program: 1h 20 min
  • Compatible Platforms: 7500 FAST Real-Time PCR System (ThermoFisher Scientific), CFX96 Real-Time PCR Cycler (BioRad)

Imegen-SARS-CoV-2 Strain Typing

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