- Quantitative assay: it allows determining the percentage of molecular chimerism in the post-transplantation period by real-time PCR (qPCR) relative quantification of the informative marker with respect to the total amount of DNA present in the sample.
- Detection limit: 0.01%
- Limit of quantification: 0.1%
- It contains a positive control that will act as a calibrator and will allow the relative quantification of the markers, without the need to use the pre-transplant recipient sample.
- It complies with the quality requirements specified by ISO 13485 and ISO 14001 in relation to the materials used in its manufacturing process.
- For use in research.
Description
Follow-up of chimerisms by real-time PCR in bone marrow transplant patients
Format
25 x 2 rxn
Technology
Real-time PCR
Reference
IMG-116 qPCR
Compatible equipment
7500 FAST Real-Time PCR System (ThermoFisher)
Real-time PCR uses a system of sequence-specific oligonucleotides and hydrolysis probes to specifically amplify targets of interest. The quantitative analysis of chimerism implements a relative calculation system implemented by the method established by Pfafl that takes into account the efficiency of the amplification system and the “cycle threshold” (Ct) values obtained for an endogenous control or calibrator, included in the kit as a positive control. From these values, the system allows the calculation of chimerism in each trial. Likewise, Imegen has developed a calculation tool to facilitate the analysis of chimerism by real-time PCR (www.imegen.es/Quimera.xls).
- Amount of DNA needed: 50 ng
- Sample type: DNA from peripheral blood or bone marrow
- Number of reactions per sample: 4
- Number of targets: 2
- Manual work time: 30-45 min
- qPCR program duration: 1h 15 min
- Compatible thermal cyclers: Real-time PCR thermal cycler with the FAMTM channel
| Reference | Kit name | Marker |
| IMG-116-2,IMG-116-18,IMG-116-3,IMG-116-4,IMG-116-5,IMG-116-6,IMG-116-7,IMG-116-8,IMG-116-9,IMG-116-10,IMG-116-11,IMG-116-12,IMG-116-13,IMG-116-14,IMG-116-17,IMG-116-20,IMG-116-21,IMG-116-23 | Imegen-Quimera SRY,Imegen-Quimera RhD,Imegen-Quimera Q116-3I,Imegen-Quimera Q116-4I,Imegen-Quimera Q116-5I,Imegen-Quimera Q116-6I,Imegen-Quimera Q116-7I,Imegen-Quimera Q116-8I,Imegen-Quimera Q116-9I,Imegen-Quimera Q116-10I,Imegen-Quimera Q116-11I ,Imegen-Quimera Q116-12I,Imegen-Quimera Q116-4D,Imegen-Quimera Q116-5D,Imegen-Quimera Q116-10D,Imegen-Quimera Q116-20I,Imegen-Quimera Q116-12D,Imegen-Quimera Q116-23I | SRY,RhD,Q116-3I,Q116-4I,Q116-5I,Q116-6I,Q116-7I,Q116-8I,Q116-9I,Q116-10I,Q116-11I ,Q116-12I,Q116-4D,Q116-5D,Q116-10D,Q116-20I,Q116-12D,Q116-23I |
Related kits
Imegen-Quimera qPCR
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