Detection of mutations W515K and W515L in the MPL gene associated with Myeloproliferative Neoplasms (MPN)

24 rxn

Real-time PCR


7500 FAST Real-Time PCR System (ThermoFisher), StepOne Real-Time PCR System (ThermoFisher), LightCycler 480 (Roche)

  • Multiplex assay that allows amplifying mutations W515L (NM_005373.2: c.1544G>T) and W515K (NM_005373.2: c.1543_1544delTGinsAA) in the MPL gene.
  • The results obtained in this test allow to confirm the diagnosis of the patient. This assay is not optimal for the study of minimal residual disease (MRD) in AML patients.
  • The detection limit has been set at 1% for each of the W515l and W515K mutations.
  • Includes an endogenous control to confirm sample quality and integrity.
  • It includes a positive control that simulates a heterozygous genotype, which guarantees the functionality of the PCR systems and which helps the interpretation in the qualitative analysis.
  • It complies with the quality requirements specified by ISO 13845 and ISO 14001 in relation to the materials used in its manufacturing process.
  • For in vitro diagnostic use.

This assay uses a combination of oligonucleotides and fluorescent hydrolysis probes in a validated assay to detect the presence of mutations in the MPL gene at the same time as the endogenous gene, b-globin, in a genomic DNA sample.

  • Amount of DNA: 100 ng total
  • Sample type: Sangre periférica (Germinal)
  • Number of reactions per sample: 2
  • Number of targets: 4
  • Manual working time: 30 min
  • Duration of the PCR program: 1h 10min
  • Compatible cyclers: real-time PCR cycler with FAMTM and VIC®


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