- Semi-quantitative analysis of the number of repetitions of the CAG expansion of the HTT gene by means of two PCRs (HTT-C and HTT-L).
- Amplification range of 100-500 bp.
- Includes a positive control at the optimal amplification concentration, in which normal alleles are present for all assays performed with the kit, to demonstrate the functionality of the prepared amplification reactions.
- Designed and validated with the same PCR program as related products for the analysis of expansions involved in other neurodegenerative diseases, so they can be analyzed together.
- It complies with the quality requirements specified by ISO 13845 and ISO 14001 in relation to the materials used in its manufacturing process.
- For in vitro diagnostic use.
Description
Detection of CAG expansion in the HTT gene associated with Huntington's disease (HD)
Format
12 rxn
Technology
Fragment Analysis
Reference
IMG-154
Compatible equipment
3730xl DNA Analyzer (ThermoFisher Scientific)
Imegen-Huntington allows analysis of CAG triplet expansions in the HTT gene associated with Huntington’s disease by conventional PCR and capillary electrophoresis.
- Amount of DNA needed: 50 ng for each amplification system
- Sample type: Peripheral blood (Germinal)
- Number of reactions per sample: 2
- Number of targets: 2 (CAG and poly-CCG)
- Manual work time: 45 minutes
- Duration of the PCR program: 2 h 15 min
- Compatible thermal cyclers: Any conventional thermal cycler
- Sequencing with compatible polymers with 6-Carboxyfluorescein (6-FAM) and NEDTM.
Imegen-Huntington
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