- Multiplex assay that allows determining the presence of normal or pathogenic alleles that give rise to the variants His63Asp (NM_000410.3:c.187C>G), p.Ser65Cys (NM_000410.3:c.193A>T) and p.Cys282Tyr ( NM_000410.3:c.845G>A) in the HFE gene, in a single PCR reaction.
- It includes a positive control that simulates a heterozygous genotype for each of the polymorphisms, which guarantees the functionality of the PCR system and helps the interpretation in the qualitative analysis.
- It complies with the quality requirements specified by ISO 13845 and ISO 14001 in relation to the materials used in its manufacturing process.
- For in vitro diagnostic use.
Description
Detection of polymorphisms p.His63Asp, p.Ser65Cys and p.Cys282Tyr in the HFE gene associated with Hemochromatosis
Format
48×3 rxn
Technology
Real-time PCR
Reference
IMG-218
Compatible equipment
7500 FAST Real-Time PCR System (ThermoFisher), StepOnePlus Real-Time PCR System (ThermoFisher), StepOne Real-Time PCR System (Thermo Fisher)
Qualitative real-time PCR assay employing a combination of oligonucleotides and fluorescent hydrolysis probes in a validated assay to simultaneously detect pathogenic p.His63Asp, p.Ser65Cys, and p.Cys282Tyr alleles and normal alleles in the coding region of the HFE gene.
- Amount of DNA needed: 150 ng total
- Sample type: Peripheral blood (Germinal)
- Number of reactions per sample: 3
- Number of targets: 6
- Manual working time: 30 min
- Duration of the PCR program: 1h
- Compatible cyclers: Real-time PCR cycler with FAMTM and VIC® channels
Imegen-HFE
Fill in the form to request this kit.
Call us at +34 963 212 340Or write to us at info@healthincode.com
