- Semi-quantitative analysis of the number of repetitions of the GAA expansion of the FXN gene by PCR.
- Amplification range of 100-500 bp.
- Includes TP-PCR systems to detect described expansions leading to amplicon sizes >500 bp.
- It complies with the quality requirements specified by ISO 9001 and ISO 14001 in relation to the materials used in its manufacturing process.
- For research use.
Description
Detection of GAA expansion in the FXN gene associated with Friedreich's ataxia (FRDA)
Format
12 rxn
Technology
Fragment Analysis
Reference
IMG-155
Compatible equipment
3730xl DNA Analyzer (ThermoFisher Scientific)
Imegen-Friedreich allows analysis of GAA triplet expansions in the FXN gene associated with Friedreich’s ataxia by conventional PCR and capillary electrophoresis. In addition, the kit offers a TP-PCR (triple repeat primed PCR) system to detect expansions not detectable with conventional PCR.
- Amount of DNA needed: 50 ng total
- Sample type: Peripheral blood (Germinal)
- Number of reactions per sample: 2
- Number of targets: 1
- Manual work time: 45 minutes
- Duration of the PCR/TP-PCR program: 2 h 15 min
- Compatible thermal cyclers: Any conventional thermal cycler
- Sequencing with polymers compatible with the label 6-Carboxyfluorescein (6-FAM)
Imegen-Friedreich
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