- Qualitative assay that allows determining the presence of the pathogenic allele c.*97G>A (20210G> A) (NM_000506.4) and the normal allele of the F2 gene in a single multiplexed PCR reaction.
- The target to detect the presence of the native genotype (20210G) is included as a control of DNA quality and integrity.
- The kit includes a positive control heterozygous for the G to A transition at position 20210 of the prothrombin gene (20210G>A).
- The detection limit for genotyping is set at 10 ng of genomic DNA.
- It complies with the quality requirements specified by ISO 9001 and ISO 14001 in relation to the materials used in its manufacturing process.
- For research use only, aimed at professionals in the molecular biology industry.
Description
Genotyping of the mutation c.*97G>A (20210G>A) of the F2 gene (Prothrombin) associated with Hereditary Thrombophilia
Format
48 rxn
Technology
Digital PCR
Reference
IMG-331
Compatible equipment
QuantStudio 3D (ThermoFisher)
Digital PCR or dPCR is a highly precise technique that uses a combination of oligonucleotides and fluorescent hydrolysis probes in an assay aimed at simultaneously amplifying and specifically detecting the targets of interest.
- Amount of DNA: 60 ng
- Sample type: Peripheral blood
- Number of reactions per sample: 1
- Number of targets: 2
- Manual working time: 30 min
- Duration of the dPCR program: 1h 20 min – 2h
- Supported Platforms: QuantStudio 3D Digital PCR System (ThermoFisher Scientific) and QX200™ Droplet Digital™ PCR System (Bio Rad)
Imegen-Factor II dPCR dry
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