- Real-time PCR assay allows determining the presence of the normal allele or the pathogenic allele p.Ala416Ser (NM000488.3:c.1246G>T) in the SERPINC1 gene associated with Cambridge II disease, in a single PCR reaction.
- High sensitivity and specificity: Discriminates in 100% of the samples the normal allele from the mutated allele associated with the Cambridge II variant.
- Validated using the same real-time PCR program as the products related to alterations in the coagulation process, so they can be analyzed together.
- It complies with the quality requirements specified by ISO 9001 and ISO 14001 in relation to the materials used in its manufacturing process.
- For research use only, aimed at professionals in the molecular biology industry.
Description
Detection of the c.1246G>T (p.Ala416Ser) allele in the SERPINC1 gene associated with Cambridge II Syndrome
Format
48 rxn
Technology
Real-time PCR
Reference
IMG-199
Compatible equipment
7500 FAST Real-Time PCR System (ThermoFisher), StepOnePlus Real-Time PCR System (ThermoFisher), StepOne Real-Time PCR System (Thermo Fisher)
Imegen-Cambridge II employs a combination of oligonucleotides and fluorescent hydrolysis probes in a real-time PCR assay to specifically discriminate targets of interest in a single PCR reaction.
- Amount of DNA needed: 50 ng total
- Sample type: peripheral (germ) blood, saliva or buccal smear
- Number of reactions per sample: 1
- Number of targets: 2
- Manual working time: 30 min
- Duration of the PCR program: 1h 15 min
- Compatible cyclers: real-time PCR cycler with FAMTM and VIC®
Imegen-Cambridge II
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