- Qualitative analysis that evaluates insertion or deletion mutations (INDELs) in exon 9 of the CALR gene in relation to the size of the amplification fragments obtained.
- Amplification range of 100-500 bp.
- According to the specified protocol, the detection limit has been established at 5%.
- It includes a positive control at the optimal amplification concentration, in which a normal allele is present, the most frequent 5-base insertion and 52-base deletion variants, as well as a single-base deletion allele demonstrating power. technique discrimination.
- It complies with the quality requirements specified by ISO 13845 and ISO 14001 in relation to the materials used in its manufacturing process.
- For in vitro diagnostic use.
Description
Detection of INDEL-type mutations in the CALR gene associated with Myeloproliferative Neoplasms (MPN)
Format
33 rxn
Technology
Fragment Analysis
Reference
IMG-237
Compatible equipment
3730xl DNA Analyzer (ThermoFisher Scientific)

Imegen-CALR allows analysis of insertions/deletions in exon 9 in the CALR gene, by means of PCR and subsequent capillary electrophoresis.
- Amount of DNA: 50 ng
- Sample type: Sangre periférica (Germinal)
- Number of reactions per sample: 1
- Number of targets: 1
- Number of targets: 30 minutos
- Number of targets: 1h 45 min
- Compatible thermal cyclers: Compatible thermal cyclers
- Sequencing with polymers compatible with the label 6-Carboxyfluorescein (6-FAM).
Related kits
Imegen-CALR
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