Hereditary OncoKitDx (without index)

Familial cancer predisposition analysis by NGS sequencing. Indexless kit to simultaneously sequence> 24 samples

48 rxn (Dilution)

NGS sequencing

IMG-326

  • Dynamic kit that updates the target genes of the panel according to scientific evidence.
  • Type of mutations analyzed: SNVs, INDELs, CNVs, ALUs and other large insertions
  • Coverage: 99.5% of the bases covered at a depth of 50X.
  • Uniformity: 99.2% of the bases covered at> 20% of the mean coverage and 90.0% of the bases covered at> 50% of the mean coverage.
  • Sensitivity:> 99.9%
  • Specificity:> 99.9%
  • It complies with the quality requirements specified by ISO 13845 and ISO 14001 regarding the materials used in its manufacturing process.

Hereditary OncoKitDx employs a library preparation technology by enzymatic fragmentation and the enrichment of the regions of interest by hybridization with capture probes. Subsequently, the massive sequencing is carried out on Illumina platforms.

Compatible sequencersIllumina iSeq, Illumina MiSeq, Illumina NextSeq500, Illumina NextSeq2000, Illumina HiSeq, Illumina NovaSeq
Number of reactions48
Number of samples per runIllumina Miseq Micro (300 cycles): 12

Illumina Miseq V2 (300 cycles): 48

Sequencing:Paired-end (2 x 150)
Amount of DNA per sample20-26 ng
Size of regions of interest184 Kb
Target genes50 genes: APC, ATM, BARD1, BMPR1A, BRCA1, BRCA2, BRIP1, CDH1, CDK4, CDKN2A, CHEK2, EPCAM, FAM175A(ABRAXAS1), FH, KIF1B, MAX, MEN1, MET, MLH1, MLH3, MRE11, MSH2, MSH3, MSH6, MUTYH, NBN, NF1, NTHL1, PALB2, PIK3CA, PMS2, POLD1, POLE, PTEN, RAD50, RAD51C, RAD51D, RB1, RET, SDHA, SDHAF2, SDHB, SDHC, SDHD, SMAD4, STK11, TMEM127, TP53, VHL, XRCC2
Type of sampleBlood or saliva

This kit is compatible with Data Genomics analysis software

Hereditary OncoKitDx (without index)

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