Action OncoKitDx (Automatic)

Analysis of the main genomic biomarkers of clinical utility, associated with the most frequent solid tumors, using a fully automated protocol and NGS sequencing.

24 rxn

NGS sequencing

IMG-365

  • Dynamic kit that updates the target genes of the panel according to scientific evidence.
  • Detection of SNVs and INDELs.
  • Analysis of fusions and rearrangements (SVs) related to targeted therapies.
  • Microsatellite Instability Detection (MSI).
  • Detection of CNVs of the selected genes, as well as losses and gains throughout the genome.
  • Pharmacogenetic analysis associated with the response to chemotherapeutic treatments.
  • Molecular marking with UMIs that increases the sensitivity of the bioinformatic analysis.
  • Software de análisis incluido: Data genomics.
  • STID: Integrated sample identification system for traceability.
  • Coverage: 99.3% of the bases covered at a depth of 100X.
  • Uniformity: 98.9% of the bases covered at> 20% of the median coverage.
  • Sensitivity:> 99%.
  • Specificity:> 99.9%.
  • The limit of detection (LOD) for SVs, SNVs, and INDELs is 5%.
  • The CNVs detection limit, with respect to the total copies of a sample, has been established at 30% when a loss occurs and at 15% for gains from a copy.
  • It complies with the quality requirements specified by ISO 13845 and ISO 14001 regarding the materials used in its manufacturing process.

Action OncoKitDx employs a technology for the preparation of libraries through mechanical fragmentation and enrichment of the regions of interest by hybridization with capture probes. This technology is fully automated. Subsequently, the massive sequencing is carried out on Illumina platforms.

Compatible sequencersIllumina NextSeq500, Illumina NextSeq2000, Illumina HiSeq, Illumina NovaSeq
Number of reactions20
Number of samples per runMid Output v2.5 kit (150 cycles): 12 samples

High Output v2.5 kit (150 cycles): 32 samples

SequencingPaired-end (2 x 75 cycles)
Amount of DNA per sample50-200 ng
Recommended DNA qualityDIN >3
Genomic region analyzed500 Kb
Target genesSNVs e INDELs: AKT1*, ALK, ARID1A, ATM, ATRX, BAP1, BRAF, BRCA1, BRCA2, CDH1, CHEK2, CTNNB1, EGFR, ERBB2, ESR1, FGFR1, FGFR4, GNA11, GNAQ, H3F3A, HIST1H3B, HIST1H3H, HRAS, IDH1, IDH2, KIT, KRAS, MAP2K1, MET, MLH1, MSH2, MSH6, MTOR, MYC, NRAS, NTRK1, NTRK2, NTRK3, PALB2, PBRM1, PDGFRA, PIK3CA, PMS2 + 5’UTR, PTEN, POLD1, POLE, RET, ROS1, SDHA, SDHB, SDHD, TERT + 5’UTR, TSC1*, TSC2*, TP53 y VHL.

* Hotspot sequencing

SVs: ALK (intron 19), ATP1B1 (introns 3 and 4), BRAF (introns 7, 8, 9 and 10), EGFR (introns 7, 23, 24 and 25), ETV6 (introns 4 and 5), FGFR2 (intron 17 and region 3’UTR), FGFR3 (intron 17 and region 3’UTR), NTRK1 (introns 8, 9, 10, 11 and 12), NTRK2 (introns 10 and 12), RET (introns 9, 10 and 11) and ROS1 (introns 31, 32, 33, 34 and 35).

DNA from peripheral blood, fresh, frozen, and paraffinized tissue

This kit is compatible with Data Genomics analysis software

Action OncoKitDx (Automatic)

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Or write to us at info@healthincode.com